Table 3 shows the ratios obtained on all Molecular Devices plate readers tested for the minimum required assay control dilutions for each assay. The fluorometric performance of the SpectraMax M2e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M3. Here, we demonstrate performance of the SpectraMax® iD3 Multi-Mode Microplate Reader for assays measuring intrinsic tryptophan fluorescence. Italic and boldface type are used for emphasis. The SpectraMax ® iD5 Multi-Mode Microplate Reader includes an absorbance detection mode optimal for protein quantitation assays. The Gemini XPS can read a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well scan modes. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. The 2-channel configuration is available with 0, 2, or 4 injectors. 专为科研检测平台量身打造的灵活且强大的小型微孔板读板机（酶标仪），具备终点法和动力学检测模式，标准配置6块滤光片，覆盖蛋白定量、细胞活力、农药残留、ELISA实验等. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. Compact and tunable absorbance microplate readers DATA SHEET. Spectranet customer portalWe would like to show you a description here but the site won’t allow us. The SpectraMax L reader is available in 1- and 2-channel (Photomultiplier Tube) configurations. This system includes the ScanLater Western Blot Detection Cartridge, ScanLater Western Blot Kit and image acquisition software powered by SoftMax Pro® Software. The CellTiter-Glo assay from Promega uses luciferase. SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. Find regional support. Cell Cytotoxicity assay procedure. Choose from a three- (M3), four- (M4), orIn this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. Our absorbance plate readers feature our PathCheck Sensor technology and. B: Selection of Excitation Wavelength. For more sample throughput, both readersThe SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. . Microplate Readers Biotek Epoch Microplate Reader. Devices’ StakMax® Microplate Stacker in a matter of minutes and . Compare plate readersFive-mode microplate reader with automatic filter identification and western blot capability The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence. Note that this procedure is only for the SpectraMax L model. We would like to show you a description here but the site won’t allow us. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. . 304 signal level saturation. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Absorbance (optical density, OD) was read at 570 nm and 605. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore ([email protected], high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. We would like to show you a description here but the site won’t allow us. A new technical approach to platelet aggregation studies. Preparation of Calibration Standards Calibration Standard (% Phosphorylated) µL Peptide Stock µL Phospho-peptide Stock 0 100 0 12. For readers without a cuvette port, these options are not shown in the software. 304 signal level saturation. These robust readers have been placed in labs from. The SpectraMax M2e is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile microplate reader capable of quantitating samples in a variety of microplate formats. Improve your lab’s efficiency, get more data, and decrease time to discovery with an automated microplate washer and reader solution: The SpectraMax® Mini Multi-Mode. 0200-6263. The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. Gemini XPS Microplate Reader (or other SpectraMax reader with fluorescence detection mode) SoftMax Pro Software; Methods Set up the instrument and software. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. This unlocks the transport slide. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. Supplier: Molecular Devices. SpectraMax iD3 Multi-Mode Microplate Reader Unparalleled performance on a personalized platform The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. Figure 1. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeMicroplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. Intended Use/Discipline:Biological. Example: [Drawer]. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . Brochure. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. Molecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. 020 fmol 0. The SpectraMax® ABS Plus Microplate Reader can run . flat meniscus), but is approx 15 mL. following formula: LLD = 3*SD blank *Concentration/(RLU sample-RLU blank). When combined with the SpectraDrop Micro-Volume Microplate, the reader can quantitate up to 64 samples with as little as 2 μL sample volume in a single read. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. Operators can conveniently select up to six wavelengths at a time for absorbance detection in the visible wavelength range of 340 nm – 850 nm. 2. keeping with best practices, reader performance should. When combined with the SpectraDrop Micro-Volume Microplate, the reader can quantitate up to 64 samples with as little as 2 µL sample volume in a. Add to Cart. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started immediately, using the preconfigured Kinetic Turbidimetric LAL protocol in SoftMax® Pro Software, with. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. The instruments affected are the SpectraMax ® L Microplate Reader and the StakMax® Microplate Handling System. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. SpectraMax iD5 Multi-Mode Microplate Reader. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Prerequisites: SoftMax Pro 7. 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. 305 reference level saturation. Molecular Devices Spectramax iD3 Microplate Reader. In addition, the SpectraMax iD5 can be expanded to. AlphaScreen assays are compatible with our SpectraMax i3x, SpectraMax i3, and SpectraMax Paradigm Multi-Mode Microplate readers with the Alphascreen Detection Cartridge options. 012 fmol 0. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. Products : General, Microplate Readers. Products. This application note describes experimental protocols that enable the detection of relatively low numbers of cells expressing Green Fluorescent Protein. The SpectraMax® iD5 multi-mode microplate reader is a complete laboratory solution to increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF) and tuneable fluorescence polarisation (FP) read modes. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has launched its SpectraMax® Mini Multi-Mode Microplate. Self Service Available. Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. The SpectraMax i3x Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. 1. Both readers feature an 8-channel read head for fast reads and a subset feature our patented PathCheck™ Sensor. Programmable liquid handling allows for perfectly timed, automated biochemical and cell-based assays. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). Table 1. Measuring platelet aggregation with a microplate reader. An ATP standard curve spanning five decades was run using the ATPlite 1step Luminescence Assay System (PerkinElmer) on the SpectraMax iD5 reader. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. 0 out of 5The SpectraMax ® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. 1. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. The advanced optical and. The SpectraMax M2 and M2e are versatile and reliable instruments that can perform a range of applications in. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. 303 unable to cal dark current. Avoid losing high signal. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. The SpectraMax® M3 Multi-Mode Microplate Reader is a UV/visible reader with dual monochromatic optics and three modes of detection for bioresearch procedures. 1. When the incubator is on, the temperature may be controlled from 2°C above ambient to 60°C. Highly customizable low volume. The system uses two scanning monochromators to determine the optimal excitation and emission settings. Download the SpectraMax Mini Reader Plug-in and associated guides. 5 12. For biological applications involving small samples, such as purificationThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance,. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. Instrument settings used with the SpectraMax M5 Microplate Reader. All stand-alone instrument functions can be controlled using the software. Users can perform spectral scans from 250 nm to 750. ELISA Plate Readers / Microplate Readers. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. 76358-624. SpectraMax M5 reader settings are shown. 0 out of 5 $ 5,999. The plate reader optics are tunable for. The SpectraMax M2 and M2e are versatile and reliable. Each sample has a discrete sample beam and reference beam so that each well is measured directly. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. 5 12. 997 (n = 3). Microplate readers from Molecular Devices are designed to provide consistent performance for many years. settings listed below for the SpectraMax iD5 reader can be used for any SpectraMax iD5 reader. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplate312 gain calibration failed. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe SpectraDrop microplate was read on the SpectraMax ® Paradigm ® Multi-Mode Microplate Reader using the Fluorescence Intensity detection cartridge (FI-FLRH) with fluorescein filters, or Tunable Wavelength Selection (TUNE) cartridge with signal/ background-optimized wavelengths. These lab workhorses are used in drug discovery, research, bioassay. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1 nm increments. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. 313 reference gain check fail. Wide range of applications. The ABS Plus reader ranges from 190 to 1000 nm in wavelength which is equivalent to having 810 free filters. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. 0 out of 5 $ 23,995 $ 22,495. Example: [Drawer]. It also integrates with the. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications. Software validation, IQ/OQ/PQ and FDA 21 CFR Part 11 compliance tools are also available. Molecular Devices SpectraMax M2 Multimode Plate Reader. Example: [Drawer]. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. Tryptophan, which excites maximally around 270-280 nm and has an emission peak near 350 nm in water, dominates the emission of proteins and is the mostPhysical specifications and backup power UPS Choices for SpectraMax® and Gemini™ microplate readers. Absorbance (optical density, OD) was read at 570 nm and 605. Download PDF versions of the SpectraMax Microplate Reader User Guides. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Sensitive fluorescent quantitation of DNA with the Quant-iT. SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Path Check Pathlength Measurement SpectraMax i3x. (A) AccuBlue Pico kit; (B) AccuClear Nano kit; (C. SpectraMax M2 and SpectraMax M2e readers can acquire absorbance as well as fluorescence data for samples by issuing a single read command in SoftMax® Pro. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. 6% for the on-board optics (monochromators). SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. Introduction. It features a modular design that allows users to add or change detection cartridges according to their needs. For more sample throughput, both readersSupplier: Molecular Devices. Page 28 Chapter 5: Read Modes and Read Types 1. 0 out of 5 $ 11,999. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. Italic and boldface type are used for emphasis. For the GMP/GLP regulated labs, the systems come equipped with our SoftMax Pro GxP. Figure 6: Transcreener ADP2 FI assay principle. 030 fmol Fluorescence Polarization (FP). The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. SpectraMax Spectrofluorometers. The calculated LOQ values were 0. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Time-. Microplate Readers Molecular Devices SpectraMax M2E Microplate Reader. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. Figure 1. 58% for the detection module and 2. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. . 2. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile. Luminescence microplate readers or luminometers are used to detect luciferase-based reporter gene assays, chemiluminescent ELISAs, BRET, and many other assays. The SpectraMax microplate readers can be used to accurately quantify ROS levels using a luminescence-based assay. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 58% for the detection module and 2. This section contains the following topics:滤光片型光吸收微孔板读板机（酶标仪），简单易用，性价比高，检测结果精确. A SpectraMax M2 microplate reader helps minimize operating time and save costs, allowing researchers to spend more time analyzing data and generating practical knowledge. SpectraMax ® M2 Multi-Mode Microplate Reader. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. Open the top cover of the instrument. keeping with best practices, reader performance should still be validated and documented regularly, especially in regulated laboratories. With optimized reagents and the industry-leading data acquisition andSpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. begin reading microplates with seven mouse clicks. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. SpectraDrop High-Throughput Screening (HTS) Kit. For readers without a cuvette port, simply check the box next to ‘PathCheck’. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. Examples: “Press carefully to engage,” “ Do not. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. SAN JOSE, CA — Molecular Devices, LLC. Win7 Manual Spectramax-L or StakMax Driver Installation after Initial Install. 7°C (gradient heating design). Here, we illustrate how the SpectraMax iD5 reader, in combination with SoftMax ® Pro Software, is used to quantitate a cellular protein sample with two different kits, Pierce BCA Protein Assay Kit and Pierce Rapid. Temperature control. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Preparation of Calibration Standards Calibration Standard (% Phosphorylated) µL Peptide Stock µL Phospho-peptide Stock 0 100 0 12. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. DNA and RNA measurements in SpectraMax® microplateSpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerAbsorbance was read at 590 nm on the SpectraMax ABS Plus Microplate Reader. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. The SpectraMax® Mini multi-mode microplate reader offers a budget friendly solution that gives you exactly what you need when you need it. When the incubator is on, the temperature may be controlled from 2°C above ambient to 60°C. 0 out of 5 $ 9,995 $ 9,495. microplate reader. The SpectraMax 340PC384 features correction for varying microplate well volumes and the elimination of standard curves based on PathCheck. Italic and boldface type are used for emphasis. Eight differentSpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. com. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). The SpectraMax M2 and M2e readers can be integrated with the StakMax® Microplate Stacker from Molecular Devices in a matter of minutes. 003 fmol 0. both cuvette-based and microplate reader applications Microplate Readers. 0 out of 5 $ 2,999-20%. The SPECTRAmax ® GEMINI-XS is a dual-monochromator microplate fluorometer system incorporating highly sensitive optics 4 that permit experimental determinations at low-level detection limits. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. limit of detection (LLD) for ATP was estimated for each microplate reader using the . Selection of Water Constant in PathCheck settings. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. The screw remains inside the instrument. Read More. Maximum signal with lowest background. 1. . Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplate 312 gain calibration failed. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance,. microplate reader. Lower limits of detection for ATP determined using the ATPlitePage 1 ® SPECTRAmax Microplate Spectrophotometer Operator’s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0085 Rev. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. The analysis capabilities of the software provide quantitation in an easy-to-read, user customizable report format. Table 1. Other technologies such as imaging. Fluorescence Microplate Reader Comparison Chart. Blank subtraction and standard curve fitting were done automatically by the software. We would like to show you a description here but the site won’t allow us. 470 nm). The SpectraMax 190 reader can be integrated with Molecular . The SpectraMax L reader is available in 1- and 2-channel (Photomultiplier Tube) configurations. Examples of results are presented below for the LMax™ II 384 dedicated, top-of-the-line microplate luminometer, the Analyst ® GT Multi-Mode, Filter-Based Instrument, and the SpectraMax ® M5 Multi-Detection, Monochromator-Based Reader with both top- and bottom-read capability. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Positions H21-K22 contained plate blanks of PBS only. SpectraMax Mini User Guide. 2. Use the provided 3. It is user upgradeable and budget friendly, and comes with a brochure that provides detailed information on its features, specifications and applications. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Devices’ StakMax® Microplate Stacker in a matter of minutes and . Find regional support. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Microplate automation and detection instruments for walk away operation and high quality results. However, microplate optimization and read height adjustment should be performed any time a new plate type—or lot of plates—or reagent volume is. The dual monochromator optics allow the. Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. MPN:SpectraMax Plus. Put 200 µL sample into one or more wells of a microplate and 200 µL buffer or solvent into one or more other wells. See SpectraMax® Microplate Reader User Manual Download Page. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingevery Gemini EM Reader. Other features and benefits include: Eliminate changing out filters. In SOFTmax PRO, set up a plate section for an excitation scan with no cutoff filter and use the medium PMT setting. This variable pathlength makes it difficult to per-NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. SpectraMax L Microplate ReaderThis UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. ; Page 2 2) All labor charges to repair the product for a period of one (1) year from the original date of delivery will be paid by Molecular Devices Corporation. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. Type: Clear: Add to Cart. Use similar settings for other Molecular Devices readers with fluorescence detection mode. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Page 35: Setting Up The Instrument SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. The ABS Plus reader ranges from 190 to 1000 nm in wavelength which is equivalent to having 810 free filters. Assays can be better optimized by scanning across. Bookings are free, but you will need to use iLab to book. Step 3: Use the Template Editor in the cell standards section of the protocol to create a template of the assay indicating the location of standards (cell numbers for each dilution. The system uses two scanning monochromators to determine the optimal excitation and emission settings. Step 2: Launch SoftMax Pro Software. 1. Offer Ends In: 2 Days 10: 40: 19. No accessories are needed and SoftMax® Pro Software. SpectraMax iD5 Multi-Mode Microplate Reader Optional, for SpectraMax iD5 reader: Fluorescence Filter with holder 565nm BW 30nm (Molecular Devices cat. The guide also covers the software features, the specifications, and the accessories of the systems. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. , a. SpectraMax® ABS Plus readers use a grating monochromator to select the exact wavelength needed for every assay. . The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Align the two slots on the bottle holder with the two pegs on the rail. The plate was placed on an shaker for 15 seconds and then incubated for 2 hours at 37°C, 5% CO 2. Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. The dual monochromator optics allow the widest range of. The SpectraMax® iD5 multi-mode microplate reader is a complete laboratory solution to increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF) and tuneable fluorescence polarisation (FP) read modes. Request a Quote!Introduction. San Jose, Calif. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. The plate was placed on an shaker for 15 seconds and then incubated for 2 hours at 37°C, 5% CO 2. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence intensity. : 2 Oct 12 Page 6 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. View the Molecular Devices website. The data suggests that there is little interference from dead cells when live cells are analyzed, however, there may be some interference from live cells when dead cells are analyzed. For a higher . 305 reference level saturation. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™certified. With optimized reagents, option to operate the. SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability.